Coding

Part:BBa_K3258007:Design

Designed by: Alec Lourenco   Group: iGEM19_Stanford   (2019-10-16)


M13 pIII neg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 754
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 723
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA was synthesized as part of a larger sequence by Twist.


Source

Synthetic sequence derived from "Negative selection and stringency modulation in phage-assisted continuous evolution."

References